Dynamic engineering of 1-alkenes biosynthesis and secretion in yeast.
Journal article, 2018

Microbial production of fatty acid-derived hydrocarbons offers a great opportunity to sustainably supply biofuels and oleochemicals. One challenge is to achieve a high production rate. Besides, low efficiency in secretion will cause high separation costs and it is therefore desirable to have product secretion. Here, we engineered the budding yeast Saccharomyces cerevisiae, to produce and secrete 1-alkenes by manipulation of the fatty acid metabolism, enzyme selection, engineering the electron transfer system and expressing a transporter. Furthermore, we implemented a dynamic regulation strategy to control the expression of membrane enzyme and transporter, which improved 1-alkene production and cell growth by relieving the possible toxicity of overexpressed membrane proteins. With these efforts, the engineered yeast cell factory produced 35.3 mg/L 1-alkenes with more than 80% being secreted. This represents a 10-fold improvement compared with earlier reported hydrocarbon production by S. cerevisiae.

Author

Yongjin Zhou

Chalmers, Biology and Biological Engineering

Yating Hu

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Zhu Zhiwei

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Verena Siewers

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

ACS Synthetic Biology

2161-5063 (eISSN)

Vol. 2 7 584-590

Driving Forces

Sustainable development

Subject Categories

Industrial Biotechnology

Infrastructure

Chalmers Infrastructure for Mass spectrometry

Roots

Basic sciences

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1021/acssynbio.7b00338

PubMed

29284088

More information

Latest update

3/7/2018 1