Iron Supplements Containing Lactobacillus plantarum 299v Increase Ferric Iron and Up-regulate the Ferric Reductase DCYTB in Human Caco-2/HT29 MTX Co-Cultures
Journal article, 2018

Several human interventions have indicated that Lactobacillus plantarum 299v (L. plantarum 299v)

increases intestinal iron absorption. The aim of the present study was to investigate possible effects

of L. plantarum 299v on the mechanisms of iron absorption on the cellular level. We have previously

shown that lactic fermentation of vegetables increased iron absorption in humans. It was revealed that

the level of ferric iron [Fe (H2O)5]2+ was increased after fermentation. Therefore, we used voltammetry

to measure the oxidation state of iron in simulated gastrointestinal digested oat and mango drinks

and capsule meals containing L. plantarum 299v. We also exposed human intestinal co-cultures of

enterocytes and goblet cells (Caco-2/HT29 MTX) to the supplements in order to study the effect

on proteins possibly involved (MUC5AC, DCYTB, DMT1, and ferritin). We detected an increase in

ferric iron in the digested meals and drinks containing L. plantarum 299v. In the intestinal cell model,

we observed that the ferric reductase DCYTB increased in the presence of L. plantarum 299v, while

the production of mucin (MUC5AC) decreased independently of L. plantarum 299v. In conclusion,

the data suggest that the effect of L. plantarum 299v on iron metabolism is mediated through driving

the Fe3+/DCYTB axis.

Lactobacillus plantarum 299v







Ann-Sofie Sandberg

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Gunilla Önning

Lunds tekniska högskola

Probi AB

Niklas Engström

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Nathalie Scheers

Chalmers, Biology and Biological Engineering, Food and Nutrition Science


2072-6643 (ISSN)

Vol. 10 Issue 12 1949

Subject Categories

Pharmacology and Toxicology


Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Areas of Advance

Life Science Engineering (2010-2018)





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