Complexation of aluminum with DNA.
Journal article, 1987

The extent of complexation of aluminum(III) with DNA (Calf thymus, Sigma type I) was estimated by means of two experimental techniques: potentiometric titration with a fluoride selective indicator electrode and dialysis followed by aluminum determination by graphite furnace AAS. Both types of experiments indicate that aluminum(III) is bound to DNA. The data are treated by assuming an ion exchange reaction with the phosphate diester groups. Using Rt to denote the concentration of these groups the values of log [AlMn-3R]/(Rt-3[AlMn-3R])[Al3+] decrease from approx. 7.6 to 5.6 when the concentration of sodium chloride is increased from 1 to 100 mM. In the pH range 4.5-5.5 the ion exchange constant increases approximately 0.5 log units. Dialysis gives lower values for the complex formation constant than potentiometry.

Osmolar Concentration

Spectrophotometry

Cations

Aluminum

Chemical Phenomena

Potentiometry

Chemistry

DNA

Author

David Dyrssen

Department of Analytical and Marine Chemistry

University of Gothenburg

Conny Haraldsson

University of Gothenburg

Department of Analytical and Marine Chemistry

Eva Nyberg

University of Gothenburg

Department of Analytical and Marine Chemistry

Margareta Wedborg

University of Gothenburg

Department of Analytical and Marine Chemistry

Journal of Inorganic Biochemistry

0162-0134 (ISSN) 18733344 (eISSN)

Vol. 29 1 67-75

Subject Categories

Analytical Chemistry

Chemical Sciences

PubMed

3559548

More information

Created

10/7/2017