Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals
Journal article, 2017

Production of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S. cerevisiae. VLCFAs can be selectively modified towards the fatty alcohol docosanol (C22H46O) by expressing a specific fatty acid reductase. Expression of this enzyme is shown to impair cell growth due to consumption of VLCFA-CoAs. We therefore implement a dynamic control strategy for separating cell growth from docosanol production. We successfully establish high-level and selective docosanol production of 83.5 mg l−1 in yeast. This approach will provide a universal strategy towards the production of similar high value chemicals in a more scalable, stable and sustainable manner.

Author

Tao Yu

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Chalmers, Biology and Biological Engineering

Nature Communications

2041-1723 (ISSN)

Driving Forces

Sustainable development

Subject Categories

Biochemistry and Molecular Biology

Biological Sciences

Microbiology

Infrastructure

Chalmers Infrastructure for Mass spectrometry

Areas of Advance

Energy

Life Science Engineering

More information

Created

1/2/2018 1