Hybrid vesicles as intracellular reactive oxygen species and nitric oxide generators
Journal article, 2019

Artificial organelles are envisioned as nanosized assemblies with intracellular biocatalytic activity to provide the host cells with non-native or missing/lost function. Hybrid vesicles loaded with glucose oxidase (NRGOx) or beta-galactosidase (NR beta-Gal) and equipped with lysosomal escape ability are assembled using phospholipids and the block copolymer poly(cholesteryl methacrylate)-block-poly(2-(dimethylamino)ethyl methacrylate). The co-localization of the building blocks and the catalytic activity of NRGOx and NR beta-Gal are illustrated. The intracellular activity of the nanoreactors in RAW 264.7 macrophages is confirmed by an enhanced reduction in viability for cells pre-incubated with NRGOx in the presence of glucose due to the generation of cytotoxic hydrogen peroxide compared to the controls. In addition, RAW 264.7 macrophages and primary human macrophages equipped with NR beta-Gal are able to intracellularly convert beta-Gal-NONOate into nitric oxide. The successful use of these hybrid vesicles to equip host macrophages with additional catalytic activity diversifies the available toolbox of nanocarriers with envisioned application in cell mimicry.

Author

Yan Zhang

Aarhus University

Noga Gal

Aarhus University

Fabian Itel

Aarhus University

Isabella N. Westensee

Aarhus University

Edit Brodszkij

Aarhus University

Daniel Mayer

University Hospital of Ulm

Steffen Stenger

University Hospital of Ulm

Miquel Castellote-Borrell

Aarhus University

Thomas Boesen

Aarhus University

Seyed Tabaei

Chalmers, Physics, Biological Physics

Fredrik Höök

Chalmers, Physics, Biological Physics

Brigitte Stadler

Aarhus University

Nanoscale

2040-3364 (ISSN)

Vol. 11 24 11530-11541

Subject Categories

Cell Biology

Immunology

Cell and Molecular Biology

DOI

10.1039/c9nr02584g

PubMed

31150038

More information

Latest update

12/16/2019