Preservation of rat skeletal muscle energy metabolism by illumination.
Artikel i vetenskaplig tidskrift, 2003

Skeletal muscle viability is crucially dependent on the tissue levels of its high energy phosphates. In this study we investigated the effect of the preservation medium Perfadex and illumination with Singlet Oxygen Energy (SOE). Singlet oxygen can be produced photochemically by energy transfer from an excited photosensitizer. The energy emitted from singlet oxygen upon relaxation to its triplet state is captured as photons at 634 nm and is here referred to as SOE. Rat hind limb rectus femoris muscles were preserved for five hours at 22 degrees C in Perfadex, saline, SOE illuminated Perfadex or SOE illuminated saline. Extracts of the muscles were analysed by 31P NMR. Data were analysed using two-way analysis of variance and are given as mean values micromol/g dry weight) +/- SEM. The ATP concentration was higher (p = 0.006) in saline groups (4.52) compared with Perfadex groups (2.82). There was no statistically significant difference in PCr between the saline groups (1.25) and Perfadex groups (0.82). However, there were higher (p = 0.003) ATP in the SOE illuminated groups (4.61) compared with the non-illuminated groups (2.73). The PCr was also higher (p < 0.0001) in the SOE illuminated groups (1.89) compared with the non-illuminated groups (0.18). In conclusion, Perfadex in this experimental model was incapable of preserving the high energy phosphates in skeletal muscle during 5 hours of ischemia. Illumination with SOE at 634 nm improved the preservation potential, in terms of a positive effect on the energy status of the muscle cell.

Sprague-Dawley

Singlet Oxygen

Phosphates

Tissue Preservation

Phosphorus Isotopes

diagnostic use

Animals

Hindlimb

Glucose-6-Phosphate

Inosine Monophosphate

Female

Citrates

Solutions

analysis

Muscle

methods

pharmacology

analysis

Phosphocreatine

Rats

analysis

analysis

Lighting

Skeletal

Adenosine Triphosphate

Energy Metabolism

Rats

Light

pharmacology

analysis

metabolism

Magnetic Resonance Spectroscopy

Författare

Ann Lindgård

Göteborgs universitet

Jonas Lundberg

Göteborgs universitet

Olivier Rakotonirainy

Anna Elander

Göteborgs universitet

Bassam Soussi

Göteborgs universitet

Life Sciences

0024-3205 (ISSN)

Vol. 72 23 2649-58

Ämneskategorier

Kirurgi

PubMed

12672510