Improved energetic recovery of skeletal muscle in response to ischemia and reperfusion injury followed by in vivo 31P-magnetic resonance spectroscopy.
Artikel i vetenskaplig tidskrift, 2002

It is of great clinical interest to improve postischemic tissue recovery during microsurgical transfers. The effect of singlet oxygen energy (SOE) as photon illumination at 634 nm on rat skeletal muscle during ischemia and postischemic reperfusion was investigated noninvasively and continuously by in vivo 31P-magnetic resonance spectroscopy (31P-MRS). A model of pedicled rat rectus femoris muscle was used, where phosphorous metabolites were followed before onset of ischemia (control), after 4 h of ischemia, and after 1 h of reperfusion. Two groups were studied: one control group (n = 10), and one SOE-treated group (n = 10). Blood perfusion was measured by laser Doppler flowmetry (LDF) during the study. After 4 h of ischemia, ATP levels were 72% and 51% of normal control values in the illuminated group and the control group, respectively (P < 0.05). After 1 h of postischemic reperfusion, phosphocreatine (PCr) recovered to 79% and adenosine triphosphate (ATP) to 71% in the illuminated group, whereas in the control group, the recovery was 57% and 51%, respectively (P < 0.05). It is concluded that singlet oxygen energy has a beneficial effect on the energy state of skeletal muscle during ischemia and postischemic reperfusion. © 2002 Wiley-Liss, Inc. MICROSURGERY 22:158–164 2002

energy

skeletal muscle

magnetic resonance spectroscopy

irradiation

ischemia

reperfision

Författare

Jonas Lundberg

Göteborgs universitet

Ann Lindgård

Göteborgs universitet

Anna Elander

Göteborgs universitet

Bassam Soussi

Göteborgs universitet

Microsurgery

0738-1085 (ISSN) 1098-2752 (eISSN)

Vol. 22 158-164

Ämneskategorier

Medicinska grundvetenskaper

DOI

10.1002/micr.21744

PubMed

12111981