Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals
Artikel i vetenskaplig tidskrift, 2017

Production of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S. cerevisiae. VLCFAs can be selectively modified towards the fatty alcohol docosanol (C22H46O) by expressing a specific fatty acid reductase. Expression of this enzyme is shown to impair cell growth due to consumption of VLCFA-CoAs. We therefore implement a dynamic control strategy for separating cell growth from docosanol production. We successfully establish high-level and selective docosanol production of 83.5 mg l−1 in yeast. This approach will provide a universal strategy towards the production of similar high value chemicals in a more scalable, stable and sustainable manner.

Författare

Tao Yu

Chalmers, Biologi och bioteknik, Systembiologi

Chalmers, Biologi och bioteknik

Nature Communications

2041-1723 (ISSN)

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Hållbar utveckling

Ämneskategorier

Biokemi och molekylärbiologi

Biologiska vetenskaper

Mikrobiologi

Infrastruktur

Chalmers infrastruktur för masspektrometri

Styrkeområden

Energi

Livsvetenskaper och teknik

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2018-01-02