Dynamic engineering of 1-alkenes biosynthesis and secretion in yeast.
Artikel i vetenskaplig tidskrift, 2018

Microbial production of fatty acid-derived hydrocarbons offers a great opportunity to sustainably supply biofuels and oleochemicals. One challenge is to achieve a high production rate. Besides, low efficiency in secretion will cause high separation costs and it is therefore desirable to have product secretion. Here, we engineered the budding yeast Saccharomyces cerevisiae, to produce and secrete 1-alkenes by manipulation of the fatty acid metabolism, enzyme selection, engineering the electron transfer system and expressing a transporter. Furthermore, we implemented a dynamic regulation strategy to control the expression of membrane enzyme and transporter, which improved 1-alkene production and cell growth by relieving the possible toxicity of overexpressed membrane proteins. With these efforts, the engineered yeast cell factory produced 35.3 mg/L 1-alkenes with more than 80% being secreted. This represents a 10-fold improvement compared with earlier reported hydrocarbon production by S. cerevisiae.

Författare

Yongjin Zhou

Chalmers, Biologi och bioteknik

Yating Hu

Chalmers, Biologi och bioteknik, Systembiologi

Zhu Zhiwei

Chalmers, Biologi och bioteknik, Systembiologi

Verena Siewers

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

ACS Synthetic Biology

2161-5063 (eISSN)

Vol. 2 7 584-590

Drivkrafter

Hållbar utveckling

Ämneskategorier

Industriell bioteknik

Infrastruktur

Chalmers infrastruktur för masspektrometri

Fundament

Grundläggande vetenskaper

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1021/acssynbio.7b00338

PubMed

29284088