Growth of a common planktonic diatom quantified using solid medium culturing
Artikel i vetenskaplig tidskrift, 2018

The ability to grow on solid culture medium is a pre-requisite for a successful microbial genetic model organism. Skeletonema marinoi, a bloom-forming, planktonic marine microalga, is widely used in ecological, evolutionary and population genetics studies. We have tested and confirmed the ability of this common organism to grow on solid culture medium (agar) under experimentally manipulated conditions. We established a protocol for quantifying growth characteristics - length of lag phase, growth rate, maximum biomass yield - on agar medium. The procedure was tested under experimental treatments and the resulting growth changes correlated with those observed in standard liquid culture. The ability to grow on solid medium broadens the use of S. marinoi as a molecular model, where agar is routinely used for various purposes (growth, selection, storage); and the possibility to quantify colony growth opens the way for high throughput, automated, or semi-automated phenotyping solutions.

Författare

Olga Kourtchenko

Göteborgs universitet

Tuomas Rajala

matematisk statistik

Anna Godhe

Göteborgs universitet

Scientific Reports

2045-2322 (ISSN)

Vol. 8 9757

Ämneskategorier

Evolutionsbiologi

Ekologi

Havs- och vattendragsteknik

DOI

10.1038/s41598-018-28129-y

PubMed

29950657

Mer information

Senast uppdaterat

2018-10-19