A Luminal Loop of Wilson Disease Protein Binds Copper and Is Required for Protein Activity
Artikel i vetenskaplig tidskrift, 2018

The copper-transporting ATPase ATP7B is essential for loading of copper ions to copper-dependent enzymes in the secretory pathway; its inactivation results in Wilson disease. In contrast to copper-ion uptake by the cytoplasmic domains, ATP7B-mediated copper-ion release in the Golgi has not been explored yet. We demonstrate here that a luminal loop in ATP7B, rich in histidine/methionine residues, binds reduced copper (Cu(I)) ions, and identified copper-binding residues play an essential role in ATP7B-mediated metal ion release. NMR experiments on short-peptide models demonstrate that three methionine and two histidine residues are specifically involved in Cu(I) ion binding; with these residues replaced by alanines, no Cu(I) ion interaction is detected. Although more than one Cu(I) ion can interact with the wild-type peptide, removing either all histidine or all methionine residues reduces the stoichiometry to one Cu(I) ion binding per peptide. Using a yeast complementation assay, we show that for efficient copper transport by full-length ATP7B, the complete set of histidine and methionine residues in the lumen loop are required. The replacement of histidine/methionine residues by alanines does not perturb overall ATP7B structure, as the localization of ATP7B variants in yeast cells matches that of the wild-type protein. Thus, in similarity to ATP7A, ATP7B also appears to have a luminal “exit” copper ion site.

Författare

Birgit Köhn

Universität Konstanz

Kumaravel Ponnandai Schanmugavel

Chalmers, Biologi och bioteknik, Kemisk biologi

Min Wu

Chalmers, Biologi och bioteknik, Kemisk biologi

M. Kovermann

Universität Konstanz

Pernilla Wittung Stafshede

Chalmers, Biologi och bioteknik, Kemisk biologi

Biophysical Journal

0006-3495 (ISSN) 1542-0086 (eISSN)

Vol. 115 6 1007-1018

Ämneskategorier

Biokemi och molekylärbiologi

Annan medicinsk grundvetenskap

Strukturbiologi

DOI

10.1016/j.bpj.2018.07.040

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Senast uppdaterat

2018-10-18