Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation
Artikel i vetenskaplig tidskrift, 2019

Background
Lignocellulosic biomass is an abundant and sustainable feedstock, which represents a promising raw material for the production of lactic acid via microbial fermentation. However, toxic compounds that affect microbial growth and metabolism are released from the biomass upon thermochemical pre-treatment. So far, susceptibility of bacterial strains to biomass-derived inhibitors still represents a major barrier to lactic acid production from lignocellulose. Detoxification of the pre-treated lignocellulosic material by water washing is commonly performed to alleviate growth inhibition of the production microorganism and achieve higher production rates.
Results
In this study, we assessed the feasibility of replacing the washing step with integrated cellular adaptation during pre-culture of Bacillus coagulans MA-13 prior to simultaneous saccharification and lactic acid fermentation of steam exploded wheat straw. Using a seed culture pre-exposed to 30% hydrolysate led to 50% shorter process time, 50% higher average volumetric and 115% higher average specific productivity than when using cells from a hydrolysate-free seed culture.
Conclusions
Pre-exposure of B. coagulans MA-13 to hydrolysate supports adaptation to the actual production medium. This strategy leads to lower process water requirements and combines cost-effective seed cultivation with physiological pre-adaptation of the production strain, resulting in reduced lactic acid production costs.

Bacillus coagulans

Wheat straw

Simultaneous saccharification and fermentation

Pre-adaptation

Hydrolysate

Författare

Martina Aulitto

Inst f Biologi, University of Naples Federico II

Chalmers, Biologi och bioteknik, Industriell bioteknik

Salvatore Fusco

Inst f Biologi, University of Naples Federico II

Chalmers, Biologi och bioteknik, Industriell bioteknik

David Benjamin Nickel

Chalmers, Biologi och bioteknik, Industriell bioteknik

Simonetta Bartolucci

Inst f Biologi, University of Naples Federico II

Patrizia Contursi

Inst f Biologi, University of Naples Federico II

Carl Johan Franzén

Chalmers, Biologi och bioteknik, Industriell bioteknik

Biotechnology for Biofuels

1754-6834 (eISSN)

Vol. 12 1 45

Förädling av inhemska förnybara råvaror till värdefulla bulk- och finkemikalier för en biobaserad samhällsekonomi: teknikutveckling, systemintegration och miljöeffekter (BioBuF)

Västra Götalandsregionen, Regionutvecklingssekretariatet, 2013-11-01 -- 2018-10-31.

Formas, 2013-06-17 -- 2018-12-31.

Bacillus coagulans som mikrobiell cellfabrik för produktion av mjölksyra från restprodukter i jord- och skogsbruk

Stiftelsen BLANCEFLOR Boncompagni Ludovisi, född Bildt, -- .

Drivkrafter

Hållbar utveckling

Ämneskategorier

Biokemikalier

Industriell bioteknik

Bioprocessteknik

Styrkeområden

Energi

Livsvetenskaper och teknik (2010-2018)

DOI

10.1186/s13068-019-1382-2

Mer information

Senast uppdaterat

2019-05-14