Evolutionary Analysis of the Bacillus subtilis Genome Reveals New Genes Involved in Sporulation
Artikel i vetenskaplig tidskrift, 2020

Bacilli can form dormant, highly resistant, and metabolically inactive spores to cope with extreme environmental challenges. In this study, we examined the evolutionary age of Bacillus subtilis sporulation genes using the approach known as genomic phylostratigraphy. We found that B. subtilis sporulation genes cluster in several groups that emerged at distant evolutionary time-points, suggesting that the sporulation process underwent several stages of expansion. Next, we asked whether such evolutionary stratification of the genome could be used to predict involvement in sporulation of presently uncharacterized genes (y-genes). We individually inactivated a representative sample of uncharacterized genes that arose during the same evolutionary periods as the known sporulation genes and tested the resulting strains for sporulation phenotypes. Sporulation was significantly affected in 16 out of 37 (43%) tested strains. In addition to expanding the knowledge base on B. subtilis sporulation, our findings suggest that evolutionary age could be used to help with genome mining.

genome mining

sporulation

Bacillus subtilis

genomic phylostratigraphy

Författare

Lei Shi

Chalmers, Biologi och bioteknik, Systembiologi

Abderahmane Derouiche

Chalmers, Biologi och bioteknik, Systembiologi

Santosh Pandit

Chalmers, Biologi och bioteknik, Systembiologi

Shadi Rahimi

Chalmers, Biologi och bioteknik, Systembiologi

Aida Kalantari

Chalmers, Biologi och bioteknik, Systembiologi

Momir Futo

Vaishnavi Ravikumar

Chalmers, Biologi och bioteknik, Systembiologi

Carsten Jers

Venkata Raghavendra Subrahmanya Sar Mokkapati

Chalmers, Kemi och kemiteknik, Energi och material, Oorganisk miljökemi 2

Kristian Vlahovicek

Ivan Mijakovic

Chalmers, Biologi och bioteknik, Systembiologi

Molecular Biology and Evolution

0737-4038 (ISSN) 1537-1719 (eISSN)

Ämneskategorier

Evolutionsbiologi

Biokemi och molekylärbiologi

Genetik

DOI

10.1093/molbev/msaa035

Mer information

Senast uppdaterat

2020-07-01