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The Journal of Maternal-Fetal & Neonatal Medicine
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group
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10.1080/14767058.2020.1777271
https://doi.org/10.1080/14767058.2020.1777271
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10.1080/14767058.2020.1777271
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Metabolomic profiles of mid-trimester amniotic fluid are not associated with subsequent spontaneous preterm delivery or gestational duration at delivery
Taylor & Francis
The Journal of Maternal-Fetal & Neonatal Medicine, 2020. doi: 10.1080/14767058.2020.1777271
Hallingström Maria
Barman Malin
Savolainen Otto
Viklund Felicia
Kacerovsky Marian
Brunius Carl
Jacobsson Bo
Amniotic fluid
gestational duration
metabolomics
mid-trimester
spontaneous preterm delivery
VoR
10.1080/14767058.2020.1777271
2020-06-16
True
www.tandfonline.com
10.1080/14767058.2020.1777271
2020-06-16
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/Title(Metabolomic profiles of mid-trimester amniotic fluid are not associated with subsequent spontaneous preterm delivery or gestational duration at delivery)
/ModDate(D:20240328160329+00'00')
/Author(Hallingstrom, M., Barman, M., Savolainen, O. et al)
/Keywords(spontaneous preterm delivery, mid-trimester, Amniotic fluid, gestational duration, metabolomics, )
/Subject(<p>Introduction:Spontaneous preterm delivery \(<37 gestational weeks\) has a multifactorial etiology with still incompletely identified pathways. Amniotic fluid is a biofluid with great potential for insights into the feto-maternal milieu. It is rich in metabolites, and metabolic consequences of inflammation is yet researched only to a limited extent. Metabolomic profiling provides opportunities to identify potential biomarkers of inflammatory conditioned pregnancy complications such as spontaneous preterm delivery.</p>\n<p>Objective:The aim of this study was to perform metabolomic profiling of amniotic fluid from uncomplicated singleton pregnancies in the mid-trimester to identify potential biomarkers associated with spontaneous preterm delivery and gestational duration at delivery. A secondary aim was to replicate previously reported mid-trimester amniotic fluid metabolic biomarkers of spontaneous preterm delivery in asymptomatic women.</p>\n<p>Method:A nested case-control study was performed within a larger cohort study of asymptomatic pregnant women undergoing mid-trimester genetic amniocentesis at 14-19 gestational weeks in Gothenburg, Sweden. Medical records were used to obtain clinical data and delivery outcome variables. Amniotic fluid samples from women with a subsequent spontaneous preterm delivery \(n = 37\) were matched with amniotic fluid samples from women with a subsequent spontaneous delivery at term \(n = 37\). Amniotic fluid samples underwent untargeted metabolomic analyses using liquid chromatography-mass spectrometry. Multivariate random forest analyses were used for data processing. A secondary targeted analysis was performed, aiming to replicate previously reported mid-trimester amniotic fluid metabolic biomarkers in women with a subsequent spontaneous preterm delivery.</p>\n<p>Results:Multivariate analysis did not distinguish the samples from women with a subsequent spontaneous preterm delivery from those with a subsequent term delivery. Neither was the metabolic profile associated with gestational duration at delivery. Potential metabolic biomarker candidates were identified from four publications by two different research groups relating mid-trimester amniotic fluid metabolomes to spontaneous PTD, of which fifteen markers were included in the secondary analysis. None of these were replicated.</p>\n<p>Conclusions:Metabolomic profiles of early mid-trimester amniotic fluid were not associated with spontaneous preterm delivery or gestational duration at delivery in this cohort.</p>)
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