%PDF-1.6
%
1 0 obj
<<
/Keywords(The pyruvate dehydrogenase complex, Free fatty acids, Redox factor, Saccharomyces cerevisiae, )
/CrossMarkDomains#5B1#5D(springer.com)
/Creator(Springer)
/ModDate(D:20240328171545+00'00')
/CreationDate(D:20201209213934+05'30')
/CrossmarkMajorVersionDate(2010-04-23)
/Subject(Background: Saccharomyces cerevisiae is being exploited as a cell factory to produce fatty acids and their derivatives as biofuels. Previous studies found that both precursor supply and fatty acid metabolism deregulation are essential for enhanced fatty acid synthesis. A bacterial pyruvate dehydrogenase \(PDH\) complex expressed in the yeast cytosol was reported to enable production of cytosolic acetyl-CoA with lower energy cost and no toxic intermediate. Results: Overexpression of the PDH complex significantly increased cell growth, ethanol consumption and reduced glycerol accumulation. Furthermore, to optimize the redox imbalance in production of fatty acids from glucose, two endogenous NAD+-dependent glycerol-3-phosphate dehydrogenases were deleted, and a heterologous NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase was introduced. The best fatty acid producing strain PDH7 with engineering of precursor and co-factor metabolism could produce 840.5 mg/L free fatty acids \(FFAs\) in shake flask, which was 83.2% higher than the control strain YJZ08. Profile analysis of free fatty acid suggested the cytosolic PDH complex mainly resulted in the increases of unsaturated fatty acids \(C16:1 and C18:1\). Conclusions: We demonstrated that cytosolic PDH pathway enabled more efficient acetyl-CoA provision with the lower ATP cost, and improved FFA production. Together with engineering of the redox factor rebalance, the cytosolic PDH pathway could achieve high level of FFA production at similar levels of other best acetyl-CoA producing pathways.)
/Author(Zhang, Y., Su, M., Qin, N. et al)
/Title(Expressing a cytosolic pyruvate dehydrogenase complex to increase free fatty acid production in Saccharomyces cerevisiae)
/CrossmarkDomainExclusive(true)
/robots(noindex)
/Producer(PDFsharp 1.50.5147 \(www.pdfsharp.com\) \(Original: Acrobat Distiller 10.1.8 \(Windows\); modified using iText 5.3.5 2000-2012 1T3XT BVBA \(SPRINGER SBM; licensed version\)\))
/doi(10.1186/s12934-020-01493-z)
/CrossMarkDomains#5B2#5D(springerlink.com)
>>
endobj
2 0 obj
<<
/Subtype/XML
/Type/Metadata
/Length 30230
>>
stream
2020-12-09T21:39:34+05:30
Springer
2020-12-10T19:13:56+01:00
2020-12-10T19:13:56+01:00
Acrobat Distiller 10.1.8 (Windows); modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version)
The pyruvate dehydrogenase complex,Free fatty acids,Redox factor,Saccharomyces cerevisiae
application/pdf
https://doi.org/10.1186/s12934-020-01493-z
BioMed Central
Microbial Cell Factories, https://doi.org/10.1186/s12934-020-01493-z
The pyruvate dehydrogenase complex
Free fatty acids
Redox factor
Saccharomyces cerevisiae
Expressing a cytosolic pyruvate dehydrogenase complex to increase free fatty acid production in Saccharomyces cerevisiae
Yiming Zhang
Mo Su
Ning Qin
Jens Nielsen
Zihe Liu
10.1186/s12934-020-01493-z
2010-04-23
true
springer.com
springerlink.com
https://doi.org/10.1186/s12934-020-01493-z
10.1186/s12934-020-01493-z
1475-2859
journal
Microbial Cell Factories
The Author(s)
2010-04-23
true
10.1186/s12934-020-01493-z
noindex
springer.com
springerlink.com
VoR
uuid:bc497500-ae9a-4d97-9a0e-493afdbadce9
uuid:87b0126d-6337-43a7-8ba5-fa7ac8faf4af
default
1
converted
uuid:f1e4cc36-6bc4-4f54-9b3f-9dbd9bd4ff4b
converted to PDF/A-2b
pdfToolbox
2020-12-09T21:42:30+05:30
2
B
Zihe Liu
http://orcid.org/0000-0002-4199-1627
http://ns.adobe.com/pdfx/1.3/
pdfx
Adobe Document Info PDF eXtension Schema
external
Mirrors crossmark:MajorVersionDate
CrossmarkMajorVersionDate
Text
external
Mirrors crossmark:CrossmarkDomainExclusive
CrossmarkDomainExclusive
Text
internal
Mirrors crossmark:DOI
doi
Text
external
Mirrors crossmark:CrosMarkDomains
CrossMarkDomains
seq Text
internal
A name object indicating whether the document has been modified to include trapping information
robots
Text
internal
ID of PDF/X standard
GTS_PDFXVersion
Text
internal
Conformance level of PDF/X standard
GTS_PDFXConformance
Text
internal
Company creating the PDF
Company
Text
internal
Date when document was last modified
SourceModified
Text
http://crossref.org/crossmark/1.0/
crossmark
Crossmark Schema
internal
Usual same as prism:doi
DOI
Text
external
The date when a publication was publishe.
MajorVersionDate
Text
internal
CrossmarkDomainExclusive
CrossmarkDomainExclusive
Text
internal
CrossMarkDomains
CrossMarkDomains
seq Text
http://prismstandard.org/namespaces/basic/2.0/
prism
Prism Schema
external
This element provides the url for an article or unit of content. The attribute platform is optionally allowed for situations in which multiple URLs must be specified. PRISM recommends that a subset of the PCV platform values, namely “mobile” and “web”, be used in conjunction with this element. NOTE: PRISM recommends against the use of the #other value allowed in the PRISM Platform controlled vocabulary. In lieu of using #other please reach out to the PRISM group at prism-wg@yahoogroups.com to request addition of your term to the Platform Controlled Vocabulary.
url
URI
external
The Digital Object Identifier for the article. The DOI may also be used as the dc:identifier. If used as a dc:identifier, the URI form should be captured, and the bare identifier should also be captured using prism:doi. If an alternate unique identifier is used as the required dc:identifier, then the DOI should be specified as a bare identifier within prism:doi only. If the URL associated with a DOI is to be specified, then prism:url may be used in conjunction with prism:doi in order to provide the service endpoint (i.e. the URL).
doi
Text
external
ISSN for an electronic version of the issue in which the resource occurs. Permits publishers to include a second ISSN, identifying an electronic version of the issue in which the resource occurs (therefore e(lectronic)Issn. If used, prism:eIssn MUST contain the ISSN of the electronic version.
issn
Text
internal
Volume number
volume
Text
internal
Issue number
number
Text
internal
Starting page
startingPage
Text
internal
Ending page
endingPage
Text
external
The aggregation type specifies the unit of aggregation for a content collection. Comment PRISM recommends that the PRISM Aggregation Type Controlled Vocabulary be used to provide values for this element. Note: PRISM recommends against the use of the #other value currently allowed in this controlled vocabulary. In lieu of using #other please reach out to the PRISM group at info@prismstandard.org to request addition of your term to the Aggregation Type Controlled Vocabulary.
aggregationType
Text
external
Title of the magazine, or other publication, in which a resource was/will be published. Typically this will be used to provide the name of the magazine an article appeared in as metadata for the article, along with information such as the article title, the publisher, volume, number, and cover date. Note: Publication name can be used to differentiate between a print magazine and the online version if the names are different such as “magazine” and “magazine.com.”
publicationName
Text
external
Copyright
copyright
Text
http://ns.adobe.com/pdf/1.3/
pdf
Adobe PDF Schema
internal
A name object indicating whether the document has been modified to include trapping information
Trapped
Text
http://ns.adobe.com/xap/1.0/mm/
xmpMM
XMP Media Management Schema
internal
UUID based identifier for specific incarnation of a document
InstanceID
URI
internal
The common identifier for all versions and renditions of a document.
DocumentID
URI
internal
The common identifier for all versions and renditions of a document.
OriginalDocumentID
URI
http://www.aiim.org/pdfa/ns/id/
pdfaid
PDF/A ID Schema
internal
Part of PDF/A standard
part
Integer
internal
Amendment of PDF/A standard
amd
Text
internal
Conformance level of PDF/A standard
conformance
Text
Springer Nature ORCID Schema
http://springernature.com/ns/xmpExtensions/2.0/
sn
authorInfo
Bag AuthorInformation
external
Author information: contains the name of each author and his/her ORCiD (ORCiD: Open Researcher and Contributor ID). An ORCiD is a persistent identifier (a non-proprietary alphanumeric code) to uniquely identify scientific and other academic authors.
AuthorInformation
http://springernature.com/ns/xmpExtensions/2.0/authorInfo/
author
Specifies the types of author information: name and ORCID of an author.
name
Text
Gives the name of an author.
orcid
URI
Gives the ORCID of an author.
http://www.niso.org/schemas/jav/1.0/
jav
NISO
external
Values for Journal Article Version are one of the following:
AO = Author’s Original
SMUR = Submitted Manuscript Under Review
AM = Accepted Manuscript
P = Proof
VoR = Version of Record
CVoR = Corrected Version of Record
EVoR = Enhanced Version of Record
journal_article_version
Closed Choice of Text
endstream
endobj
3 0 obj
<<
/Contents 4 0 R
/Thumb 5 0 R
/Type/Page
/PieceInfo
<<
/InDesign
<<
/LastModified
/PageWidthList
<<
/0 595.276
>>
/NumberofPages 1
/OriginalDocumentID
/DocumentID
/PageUIDList
<<
/0 2888
>>
>>
>>
/Resources 6 0 R
/Annots 7 0 R
/CropBox[0 0 595.276 790.866]
/Parent 8 0 R
/Rotate 0
/MediaBox[0 0 595.276 790.866]
/Group
<<
/CS/DeviceRGB
/S/Transparency
>>
>>
endobj
4 0 obj
<<
/Length 15439
/Filter/FlateDecode
>>
stream
x}$q-Y&0iD/ߙe +uG37m<8}1U={\άxGTW'~}g_뻓_:ݽzsZ2+MyEWJ{כɗ&,_o:]߽@=V*.W_T^ߜ.b{v{ׯN#nvS3\_-2N٘?yu,IcI{'~zOVwyJؽ_r4u*BW*`sZ{S;]j8lӕ_=n?]UX+,ޜ.b}ӵ۰Gl%/ Sy_"=Y$@nge
_.~1>|^}ͭ
q͓idz[17_|7qKf0%qS}.cqq#`;n{խet4SsLQgH7./7nAx.6O >0=Y-U_ﻒƄa_ l
]7W:?ŋnU <`qn<]w+WIѴ}4*o\6W^R6 6 3-5^p6a5)zֽvѥi],eQ>|cM7=cr}=U="ϯOuX|1
:
8oˑsVLwC[3d:9W
xsaUC~gi"}3bƳW1ͧ_g?1]Ad5S4hFs'`{<:pW5Hj:l&J.5_&Wc?t&[~G{ޏ-4oz7]~o_`z+hO%
A5_! |f4lo 2-I a*+qE=kBq6tuðXBcs|wbKfLbSCm~˼'S7Ntcl&ЯB^0Wҙ_UD~Ô:f^zVԁ(6\tY;,6{sIP
/Nٟ_|o).\~Sh%yhqئYvi'Ho7eF9г9#Eh7]yntmɌEŭx>9qpO{)ʇVOhht/N^z _YY2b4RZVx+ᷥVҩq?B_Aa7/ Fys1 s_T>q]5WR7A̘/yI-ʀ5^ݍ(_
<@ ٰ(u<^#fӈIȂ#fU(efSƬd* JAA {&c{ٱE|]d{!Ff\ߗN[e*fim
U8+Cwd H$ˈķvsA9cM#NQ盎VԉjDT.|`c1m022 `kHsA$O5V|"AH#x$"~*?J-q/|mx, ,`
,Trl߿{XZ-kاJ1X~~M(pEQ#Yu^UlاAdHe,J࿏hNW@RUD]YHit
= fsjFVKRG5ёHorK<,qМ7DWU뚲&Aard0\d 2c+kl>F0&N]~-оx#3C31D!O{TgfBC ZnF%b>? Lc8ݵ%'~o۩Ip:FO?Oi"OydكqNm2jpŨq0MYA
6)
g٧$]#'ukӳ31B2^F+d%//XQo
cxJUL'bROԭ'@4IE<ݗeT .Tw틴wpu\r"kaK{[<{R0*(zf*GdHv(1AN׃݃5Iio<
&r܁ķA^EL|AgAb09pdǪg#8[1XƋ'q\jߧ8ӨlkLQZ;Uc&+^!5>_ ު5ְtPQl v%Qr^@0i^'?we?ð<Ѓai;bؑ=鿗W٤81h,JP멲QfN8xy/_<ϡ ӈBLP 3h{BY *WXia P˧S 9Ce%V`hnvF>B }$h
VdI{KZH7L۸|ĝsw^o\뫖gV#/fezǂ'{U aʚpONUUEݵz"͈%j$^
q<}CIn,yڻ
-7&v]d}By3{