A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood
Artikel i vetenskaplig tidskrift, 2021

We report a microfluidic sandwich immunoassay constructed around a dual-giant magnetoresistance (GMR) sensor array to quantify the heart failure biomarker NT-proBNP in human plasma at the clinically relevant concentration levels between 15 pg/mL and 40 ng/mL. The broad dynamic range was achieved by differential coating of two identical GMR sensors operated in tandem, and combining two standard curves. The detection limit was determined as 5 pg/mL. The assay, involving 53 plasma samples from patients with different cardiovascular diseases, was validated against the Roche Cobas e411 analyzer. The salient features of this system are its wide concentration range, low detection limit, small sample volume requirement (50 μL), and the need for a short measurement time of 15 min, making it a prospective candidate for practical use in point of care analysis.

POCT

GMR

Microfluidic

Biosensor

NT-proBNP

Författare

Fanda Meng

Shandong First Medical University

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Shandong Qianfoshan Hospital

Weisong Huo

Dongguan Bosh Biotechnologies

Jie Lian

People's Public Security University of China

Lei Zhang

Dongguan Bosh Biotechnologies

Xizeng Shi

Dongguan Bosh Biotechnologies

Aldo Jesorka

Chalmers, Kemi och kemiteknik

Yunhua Gao

Chinese Academy of Sciences

Analytical and Bioanalytical Chemistry

1618-2642 (ISSN) 1618-2650 (eISSN)

Vol. 413 11 2943-2949

Ämneskategorier

Medicinsk laboratorie- och mätteknik

Analytisk kemi

Biomedicinsk laboratorievetenskap/teknologi

DOI

10.1007/s00216-021-03227-5

PubMed

33624128

Mer information

Senast uppdaterat

2021-05-28