Strategies for Improving the Protein Yield in pH-Shift Processing of Ulva lactuca Linnaeus: Effects of Ulvan Lyases, pH-Exposure Time, and Temperature
Artikel i vetenskaplig tidskrift, 2019

Globally, there is a need for novel vegetarian protein sources. We recently showed that the pH-shift process, using alkaline protein solubilization followed by isoelectric precipitation, is an efficient way to produce extracts with high protein concentrations from Ulva lactuca (>50% on a dry matter basis). However, the total protein yield was low, and to improve this, the effects of adding ulvan lyase, preincubating the seaweed homogenate at pH 8.5 and using different protein extraction temperatures (8 °C, RT and 40 °C), were evaluated in this study. Addition of ulvan lyase reduced protein solubility but increased the precipitation. Incubation at pH 8.5, without ulvan lyase added, significantly increased both protein solubility and precipitation at 8 °C and RT. Temperature per se had no effect on protein solubility, while protein precipitation increased with decreasing temperature. Highest protein yield (29%) was achieved when keeping the process at 8 °C with a preincubation step at pH 8.5 for 1 h. By these process modifications, the yield was 3.2 times higher than achieved by the control process (9.2%).

Protein extraction

Ulvan lyase

Protein yield

pH-shift

Seaweed

Ulva lactuca

Författare

[Person 9c149b5d-2d1f-479a-8312-32e515a259ff not found]

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

[Person 3af31757-8422-4fce-9412-a76e377558f4 not found]

Chalmers, Biologi och bioteknik, Industriell bioteknik

[Person dffcab4b-1dd0-4716-a6a9-a7d2095f063b not found]

Göteborgs universitet

[Person bb48d65a-d4dd-4e03-87e3-9457a4fb1807 not found]

Göteborgs universitet

[Person 124ee3c3-efcd-4848-9512-7ba3b9fbaaa2 not found]

Chalmers, Biologi och bioteknik, Industriell bioteknik

[Person 0f400a0e-2f67-46b9-a066-01f373552732 not found]

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

ACS Sustainable Chemistry & Engineering

2168-0485 (eISSN)

Vol. 7 15 12688-12691

Produktionssystem för alger med högvärdiga tillämpningar

Stiftelsen för Strategisk forskning (SSF), 2015-01-01 -- 2019-12-31.

Drivkrafter

Hållbar utveckling

Ämneskategorier

Livsmedelsteknik

Strukturbiologi

Annan industriell bioteknik

DOI

10.1021/acssuschemeng.9b02781

Mer information

Senast uppdaterat

2019-10-21