Protein adsorption and desorption on lipid bilayers
Artikel i vetenskaplig tidskrift, 2010
The protein surface usually exhibits one or a few charged spots. If a lipid bilayer contains a significant amount of lipids with oppositely charged head groups, protein adsorption on a bilayer may be energetically favourable due to the protein-lipid electrostatic interaction. The specifics of this case are that the lipids are highly mobile and the protein adsorption is accompanied by the redistribution of lipids between the areas covered and not covered by protein. We present a kinetic model illustrating that this effect is especially interesting if the fraction of the surface covered by charged lipids is relatively low. In this situation, with increasing protein coverage, the protein desorption rate constant rapidly increases while the adsorption rate constant drops, so that there is critical fraction of the area covered by protein. Adsorption above this fraction is hindered both kinetically and thermodynamically.
charged
monte-carlo simulations
solid-surfaces
membranes
Lipids
Adsorption and desorption kinetics
Lipid
macromolecules
phospholipid-bilayers
redistribution
electrostatics
Proteins
excluded surface-area
kinetics
Electrostatic interactions
aggregation
qcm-d
Författare
Vladimir Zhdanov
Chalmers, Teknisk fysik, Kemisk fysik
Bengt Herbert Kasemo
Chalmers, Teknisk fysik, Kemisk fysik
Biophysical Chemistry
0301-4622 (ISSN) 18734200 (eISSN)
Vol. 146 2-3 60-64Ämneskategorier
Biokemi och molekylärbiologi
DOI
10.1016/j.bpc.2009.10.005