Optical mapping of single DNA molecules in nanochannels: A novel method for identification and characterization of antibiotic resistance
Paper i proceeding, 2015

The use, and overuse, of antibiotics has during the last decade led to a dramatic increase in antibiotic resistance and there is a crying need for novel methods for fast identification of antibiotic resistance genes. We here demonstrate how our previously developed assay for optical mapping of DNA in nanochannels can be used for characterization of resistance genes located on plasmid DNA from bacteria. The assay requires extremely small sample volumes and does neither rely on PCR, nor culturing of bacteria, which greatly reduces the time for analysis.

Plasmid DNA

Nanofluidics

Antibiotic resistance

Optical mapping

Författare

Lena Nyberg

Chalmers, Biologi och bioteknik, Kemisk biologi

Gustav Emilsson

Chalmers, Teknisk fysik, Bionanofotonik

A. Nilsson

Lunds universitet

E. Lagerstedt

Lunds universitet

C. Noble

Lunds universitet

Liselott Svensson-Stadler

Göteborgs universitet

N. Karami

Göteborgs universitet

Fei Sjöberg

Göteborgs universitet

Edward R.B. Moore

Göteborgs universitet

Joachim Fritzsche

Chalmers, Teknisk fysik, Kemisk fysik

Erik Kristiansson

Göteborgs universitet

T. Ambjornsson

Lunds universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

1045-1047
978-09-79-80647-6 (ISBN)

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik (2010-2018)

Ämneskategorier

Biokemi och molekylärbiologi

ISBN

978-09-79-80647-6

Mer information

Senast uppdaterat

2018-03-02