Disposable microfluidic chip with integrated light sheet illumination enables diagnostics based on membrane vesicles
Paper i proceeding, 2013
Cell-derived membrane vesicles that are released in body fluids are emerging as potential non-invasive biomarkers for diseases like cancer. Techniques capable of measuring the size and concentration of such membrane vesicles directly in body fluids are urgently needed. Here we report on a microfluidic chip with integrated light sheet illumination, and demonstrate accurate fluorescence Single Particle Tracking measurements of the size and concentration of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours.
Diagnostics
Single Particle Tracking
Light sheet microscopy
Lab-on-a-chip
Författare
Hendrik Deschout
Universiteit Gent
K. Raemdonck
Universiteit Gent
S. Stremersch
Universiteit Gent
P. Maoddi
Ecole Polytechnique Federale de Lausanne (EPFL)
G. Mernier
Ecole Polytechnique Federale de Lausanne (EPFL)
P. Renaud
Ecole Polytechnique Federale de Lausanne (EPFL)
S. Jiguet
Ecole Polytechnique Federale de Lausanne (EPFL)
A. Hendrix
Universiteit Gent
M. Bracke
Universiteit Gent
R. Van den Broecke
Universiteit Gent
Magnus Röding
Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik
Göteborgs universitet
Mats Rudemo
Göteborgs universitet
Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik
J. Demeester
Universiteit Gent
S. De Smedt
Universiteit Gent
F. Strubbe
Universiteit Gent
K. Neyts
Universiteit Gent
Kevin Braeckmans
Universiteit Gent
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013
Vol. 3 2010-2012
Ämneskategorier
Biokemi och molekylärbiologi