Bioimaging TOF-SIMS: High resolution 3D imaging of single cells.
Artikel i vetenskaplig tidskrift, 2007
The distribution of phosphocholine ions (m/z 184, m/z 86), sodium ions, and potassium ions in thyroid tumor cells was analyzed by imaging TOF-SIMS. Repeated sputtering with a C(60) (+) source and subsequent analysis with a Bi(3) (+) gun produced a series of 138 images that were stacked to make a 3D display of the chemistry of cells. Phosphocholine was seen in the plasma membrane (m/z 184) and intracellular membranes (m/z 86). The different fragmentation of the phospholipid probably reflects the chemical composition of membranes at these sites. High intensity of secondary ion signals of potassium was seen in membrane-encompassed cellular compartments. The data indicate that potassium ions are compartmentalized in thyroid tumor cells.
analysis
analysis
Cell Line
Potassium
Humans
Tumor
Three-Dimensional
analysis
Ions
Phosphorylcholine
Imaging
analysis
Sodium
Mass Spectrometry
Författare
Håkan Nygren
Göteborgs universitet
Birgit Hagenhoff
Per Malmberg
Göteborgs universitet
Mikael Nilsson
Göteborgs universitet
Katrin Börner
Göteborgs universitet
Microscopy Research and Technique
1059-910X (ISSN)
Vol. 70 11 969-74Ämneskategorier
Cell- och molekylärbiologi
Annan medicinsk grundvetenskap
DOI
10.1002/jemt.20502
PubMed
17661396