An Optofluidic Temperature Probe
Artikel i vetenskaplig tidskrift, 2013

We report the application of a microfluidic device for semi-contact temperature measurement in picoliter volumes of aqueous media. Our device, a freely positionable multifunctional pipette, operates by a hydrodynamic confinement principle, i.e., by creating a virtual flow cell of micrometer dimensions within a greater aqueous volume. We utilized two fluorescent rhodamines, which exhibit different fluorescent responses with temperature, and made ratiometric intensity measurements. The temperature dependence of the intensity ratio was calibrated and used in a model study of the thermal activation of TRPV1 ion channels expressed in Chinese hamster ovary cells. Our approach represents a practical and robust solution to the specific problem of measuring temperature in biological experiments in vitro, involving highly localized heat generation, for example with an IR-B laser.

microfluidic device

multifunctional pipette

optofluidic

temperature sensing

Rhodamine B

microthermometer

Rhodamine 6G

semi-contact

TRPV1

Författare

Ilona Wegrzyn

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Alar Ainla

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Gavin Jeffries

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Aldo Jesorka

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Sensors

1424-8220 (ISSN) 1424-3210 (eISSN)

Vol. 13 4289-4302

Ämneskategorier

Kemi

DOI

10.3390/s130404289