A Method for Investigation of Size-Dependent Protein Binding to Nanoholes Using Intrinsic Fluorescence of Proteins
Artikel i vetenskaplig tidskrift, 2017

We have developed a novel method to study the influence of surface nanotopography on human fibrinogen adsorption at a given surface chemistry. Well-ordered arrays of nanoholes with different diameters down to 45 nm and a depth of 50 nm were fabricated in silicon by electron beam lithography and reactive ion etching. The nanostructured chip was used as a model system to understand the effect of size of the nanoholes on fibrinogen adsorption. Fluorescence imaging, using the intrinsic fluorescence of proteins, was used to characterize the effect of the nanoholes on fibrinogen adsorption. Atomic force microscopy was used as a complementary technique for further characterization of the interaction. The results demonstrate that as the size of the nanoholes is reduced to 45 nm, fibrinogen adsorption is significantly increased.

adsorption

water

nanoparticles

ultraviolet fluorescence

activation

Författare

Bita Malekian

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Lunds universitet

Ivan Maximov

Lunds universitet

Rainer Timm

Lunds universitet

Tommy Cedervall

Lunds universitet

Dan Hessman

Lunds universitet

ACS Omega

24701343 (eISSN)

Vol. 2 8 4772-4778

Ämneskategorier

Kemiteknik

DOI

10.1021/acsomega.7b00241

Mer information

Senast uppdaterat

2023-03-21