Single neuronal network analysis using microdroplet concentrator coupled mass spectrometry: Studying neurotransmitter variances for the treatment of brain disorders

I propose to develop a unique platform to study Glutamatergic, Dopaminergic and Acetylcholine neurotransmission,under modulation of antipsychotic drugs. Through fluidic isolation of pyramidal neurons for targeted region dosing, we will to elucidate key modes of action not only from a targeted cell, but from the extended network, to which the cell is directly or indirectly connected. I aim to create an analytical platform, to deliver analytes, while simultaneously monitoring the extracellular environment, through regular sample collection, concentration and storage (for later analysis). Nano-esi mass spectrometry will be utilized to detect and quantify the responses. Using a free standing microfluidic platform the response of a single neuron or neuronal cluster can be isolated, enabling interpretation of drug interactions and analysis of their metabolites, without the detrimental effect of ensemble averaging. Through precise fluid handling schemes, diffusional mixing can be minimized, allowing for collection and droplet formation without dramatic dilution. Through droplet formation and passive concentration, smaller neurotransmitter concentrations can be detected, while maintaining high temporal resolution, crucial in investigating pharmacokinetics with higher fidelity.

Participants

Gavin Jeffries (contact)

Forskarassistent vid Physical Chemistry

Funding

Swedish Research Council (VR)

Funding Chalmers participation during 2013–2016

Publications

More information

Latest update

2015-09-04