Deletion of the G protein-coupled receptor 30 impairs glucose tolerance, reduces bone growth, increases blood pressure, and eliminates estradiol-stimulated insulin release in female mice.
Journal article, 2009
In vitro studies suggest that the G protein-coupled receptor (GPR) 30 is a functional estrogen receptor. However, the physiological role of GPR30 in vivo is unknown, and it remains to be determined whether GPR30 is an estrogen receptor also in vivo. To this end, we studied the effects of disrupting the GPR30 gene in female and male mice. Female GPR30((-/-)) mice had hyperglycemia and impaired glucose tolerance, reduced body growth, increased blood pressure, and reduced serum IGF-I levels. The reduced growth correlated with a proportional decrease in skeletal development. The elevated blood pressure was associated with an increased vascular resistance manifested as an increased media to lumen ratio of the resistance arteries. The hyperglycemia and impaired glucose tolerance in vivo were associated with decreased insulin expression and release in vivo and in vitro in isolated pancreatic islets. GPR30 is expressed in islets, and GPR30 deletion abolished estradiol-stimulated insulin release both in vivo in ovariectomized adult mice and in vitro in isolated islets. Our findings show that GPR30 is important for several metabolic functions in female mice, including estradiol-stimulated insulin release.
veterinary
Animals
Glucose Tolerance Test
metabolism
Male
Mice
Pregnancy
genetics
Gene Deletion
Glucose Intolerance
Bone Development
Inbred C57BL
Islets of Langerhans
Estradiol
Knockout
Tissue Distribution
Sex Characteristics
genetics
metabolism
Mice
Female
RNA
pharmacology
Mice
Messenger
genetics
Receptors
genetics
G-Protein-Coupled
Blood Pressure
metabolism
physiology
secretion
Insulin
Author
Ulrika E A Mårtensson
S Albert Salehi
Sara H Windahl
University of Gothenburg
Maria F Gomez
Karl Swärd
Joanna Daszkiewicz-Nilsson
Anna Wendt
Niklas Andersson
University of Gothenburg
Per Hellstrand
Per-Olof Grände
Christer Owman
Clifford J Rosen
Martin L Adamo
Ingmar Lundquist
Patrik Rorsman
Bengt-Olof Nilsson
Claes Ohlsson
University of Gothenburg
Björn Olde
L M Fredrik Leeb-Lundberg
Endocrinology
1945-7170 (ISSN)
Vol. 150 2 687-98Subject Categories
Endocrinology and Diabetes
DOI
10.1210/en.2008-0623
PubMed
18845638