A microfluidic device for reversible environmental changes around single cells using optical tweezers for cell selection and positioning
Journal article, 2010

Cells naturally exist in a dynamic chemical environment, and therefore it is necessary to study cell behaviour under dynamic stimulation conditions in order to understand the signalling transduction pathways regulating the cellular response. However, until recently, experiments looking at the cellular response to chemical stimuli have mainly been performed by adding a stress substance to a population of cells and thus only varying the magnitude of the stress. In this paper we demonstrate an experimental method enabling acquisition of data on the behaviour of single cells upon reversible environmental perturbations, where microfluidics is combined with optical tweezers and fluorescence microscopy. The cells are individually selected and positioned in the measurement region on the bottom surface of the microfluidic device using optical tweezers. The optical tweezers thus enable precise control of the cell density as well as the total number of cells within the measurement region. Consequently, the number of cells in each experiment can be optimized while clusters of cells, that render subsequent image analysis more difficult, can be avoided. The microfluidic device is modelled and demonstrated to enable reliable changes between two different media in less than 2 s. The experimental method is tested by following the cycling of GFP-tagged proteins (Mig1 and Msn2, respectively) between the cytosol and the nucleus in Saccharomyces cerevisiae upon changes in glucose availability.

Author

Emma Eriksson

University of Gothenburg

Kristin Sott

SuMo Biomaterials

University of Gothenburg

Fredrik Lundqvist

University of Gothenburg

Martin Sveningsson

University of Gothenburg

Jan Scrimgeour

Dag Hanstorp

University of Gothenburg

Mattias Goksör

University of Gothenburg

Annette Graneli

University of Gothenburg

Lab on a Chip - Miniaturisation for Chemistry and Biology

1473-0197 (ISSN) 1473-0189 (eISSN)

Vol. 10 5 617-625

Subject Categories

Other Physics Topics

Condensed Matter Physics

DOI

10.1039/b913587a

More information

Created

10/7/2017