Adaption of the xylose fermenting yeast Saccharomyces cerevisiae F12 for improving ethanol production in different fed-batch SSF processes
Journal article, 2010

An efficient fermenting microorganism for bioethanol production from lignocellulose is highly tolerant to the inhibitors released during pretreatment and is able to ferment efficiently both glucose and xylose. In this study, directed evolution was employed to improve the xylose fermenting Saccharomyces cerevisiae F12 strain for bioethanol production at high substrate loading. Adapted and parental strains were compared with respect to xylose consumption and ethanol production. Adaption led to an evolved strain more tolerant to the toxic compounds present in the medium. When using concentrated prehydrolysate from steam-pretreated wheat straw with high inhibitor concentration, an improvement of 65 and 20% in xylose consumption and final ethanol concentration, respectively, were achieved using the adapted strain. To address the need of high substrate loadings, fed-batch SSF experiments were performed and an ethanol concentration as high as 27.4 g/l (61% of the theoretical) was obtained with 11.25% (w/w) of water insoluble solids (WIS).

Fed-batch

Xylose

S. cerevisiae

Bioethanol

Evolutionary engineering

Author

Elia Tomas-Pejo

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas (Ciemat)

M. Ballesteros

Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas (Ciemat)

J.M Oliva

Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas (Ciemat)

Lisbeth Olsson

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Journal of Industrial Microbiology and Biotechnology

1367-5435 (ISSN) 1476-5535 (eISSN)

Vol. 37 11 1211-1220

Subject Categories

Industrial Biotechnology

DOI

10.1007/s10295-010-0768-8

More information

Latest update

7/22/2019