Studies of glycolytic flux regulation/control using permeabilised yeast cells
Conference contribution, 2010

In this work the correlation between levels of adenosine nucleotides and glycolytic flux was studied using a permeabilised cell system by experiments and in silico simulations using a kinetic model of glycolysis. Experimentally, using a flow-through chamber, the ATP level was correlated to glycolytic flux by a parabolic relation with a maximum at 2-3 mM of ATP. The kinetic model did not describe this parabolic correlation; at high ATP levels above 4 mM the flux was further increased. Thus, other mechanisms than described by the kinetic equations used in the model are important at high ATP levels to reduce the glycolytic flux.


Eva Albers

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Proceedings of 14th Workshop of International Study Group for Systems Biology (ISGSB)


Subject Categories

Biochemistry and Molecular Biology


Areas of Advance

Life Science Engineering (2010-2018)



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