Studies of glycolytic flux regulation/control using permeabilised yeast cells
Conference contribution, 2010

In this work the correlation between levels of adenosine nucleotides and glycolytic flux was studied using a permeabilised cell system by experiments and in silico simulations using a kinetic model of glycolysis. Experimentally, using a flow-through chamber, the ATP level was correlated to glycolytic flux by a parabolic relation with a maximum at 2-3 mM of ATP. The kinetic model did not describe this parabolic correlation; at high ATP levels above 4 mM the flux was further increased. Thus, other mechanisms than described by the kinetic equations used in the model are important at high ATP levels to reduce the glycolytic flux.

Author

Eva Albers

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Proceedings of 14th Workshop of International Study Group for Systems Biology (ISGSB)

97-102

Subject Categories

Biochemistry and Molecular Biology

Microbiology

Areas of Advance

Life Science Engineering (2010-2018)

ISBN

978-5-98036-015-3

More information

Created

10/7/2017