Michler's Hydrol Blue: A Sensitive Probe for Amyloid Fibril Detection
Journal article, 2011

Michlers hydrol blue (MHB) is investigated with respect to photophysical properties in varied solvent environment and when bound to insulin and lysozyme fibrils. The MHB chromophore is shown to act like a molecular rotor and bind well to amyloid fibrils, where it exhibits a characteristic red-shift in its excitation spectrum and an increase in the emission quantum yield upon binding. MHB is more sensitive to environmental changes than Thioflavin T (ThT) and furthermore, in contrast to the latter amyloid probe, can differentiate between insulin and lysozyme fibrils by a more red-shifted excitation spectrum for insulin fibrils. To support the experimental observations, time-dependent density functional theory (TDDFT) calculations were performed on MHB at several levels of theory. The predicted changes of spectral properties as a function of the environment are in good agreement with the experimental results. Linear dichroism (LD) is used to determine the orientation of the MHB within the fibrils. It was shown through LD and molecular modeling that MHB aligns itself preferentially parallel with the amyloid fiber at an angle of 14 degrees-22 degrees to the fibril axis and along the grooves of the beta-sheet.

fluorescent properties

molecular rotors

protein

excited-state

atomic-force microscopy

congo red

alzheimers-disease

insulin

thioflavin-t

linear dichroism

Author

Catherine Kitts

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Tamas Beke-Somfai

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Bengt Nordén

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Biochemistry

0006-2960 (ISSN) 1520-4995 (eISSN)

Vol. 50 17 3451-3461

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Energy

Life Science Engineering (2010-2018)

Materials Science

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1021/bi102016p

More information

Created

10/8/2017