A new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurement
Journal article, 2011

This article presents a new design of flow-orientation device for the study of bio-macromolecules, including DNA and protein complexes, as well as aggregates such as amyloid fibrils and liposome membranes, using Linear Dichroism (LD) spectroscopy. The design provides a number of technical advantages that should make the device inexpensive to manufacture, easier to use and more reliable than existing techniques. The degree of orientation achieved is of the same order of magnitude as that of the commonly used concentric cylinders Couette flow cell, however, since the device exploits a set of flat strain-free quartz plates, a number of problems associated with refraction and birefringence of light are eliminated, increasing the sensitivity and accuracy of measurement. The device provides similar shear rates to those of the Couette cell but is superior in that the shear rate is constant across the gap. Other major advantages of the design is the possibility to change parts and vary sample volume and path length easily and at a low cost.

SPECTROSCOPY

LIPID RAFTS

GELS

DNA

MEMBRANE

BINDING

Author

Johan Lundahl

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Catherine Kitts

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Bengt Nordén

Chalmers, Chemical and Biological Engineering, Physical Chemistry

The Analyst

0003-2654 (ISSN) 1364-5528 (eISSN)

Vol. 136 16 3303-3306

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Energy

Life Science Engineering (2010-2018)

Materials Science

Subject Categories

Chemical Sciences

DOI

10.1039/c0an00455c

More information

Created

10/6/2017