Modification and expulsion of keratins by human epidermal keratinocytes upon hapten exposure in vitro.
Journal article, 2011

Allergic contact dermatitis is the most prevalent form of human immunotoxicity. It is caused by reactive low molecular weight chemicals, that is, haptens, coming in contact with the skin where hapten-peptide complexes are formed, activating the immune system. By using sensitizing fluorescent thiol-reactive haptens, that is, bromobimanes, we show how keratinocytes respond to hapten exposure in vitro and reveal, for the first time in a living system, an exact site of haptenation. Rapid internalization and reaction of haptens with keratin filaments were visualized. Subsequently, keratinocytes respond in vitro to hapten exposure by release of membrane blebs, which contain haptenated keratins 5 and 14. Particularly, cysteine 54 of K5 was found to be a specific target. A mechanism is proposed where neoepitopes, otherwise hidden from the immune system, are released after hapten exposure via keratinocyte blebbing. The observed expulsion of modified keratins by keratinocytes in vitro might play a role during hapten sensitization in vivo and should be subject to further investigations.


Brigitte Bauer

University of Gothenburg

Sofia Andersson

University of Gothenburg

Anna-Lena Stenfeldt

University of Gothenburg

Carl Simonsson

University of Gothenburg

Jörgen Bergström

University of Gothenburg

Marica B Ericson

University of Gothenburg

Charlotte A Jonsson

University of Gothenburg

Kerstin S Broo

Chemical Research in Toxicology

0893-228X (ISSN) 1520-5010 (eISSN)

Vol. 24 5 737-43

Subject Categories

Other Basic Medicine

Other Chemistry Topics





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