Time-Resolved WAXS Reveals Accelerated Conformational Changes in Iodoretinal-Substituted Proteorhodopsin.
Journal article, 2011

Time-resolved wide-angle x-ray scattering (TR-WAXS) is an emerging biophysical method which probes protein conformational changes with time. Here we present a comparative TR-WAXS study of native green-absorbing proteorhodopsin (pR) from SAR86 and a halogenated derivative for which the retinal chromophore has been replaced with 13-desmethyl-13-iodoretinal (13-I-pR). Transient absorption spectroscopy differences show that the 13-I-pR photocycle is both accelerated and displays more complex kinetics than native pR. TR-WAXS difference data also reveal that protein structural changes rise and decay an order-of-magnitude more rapidly for 13-I-pR than native pR. Despite these differences, the amplitude and nature of the observed helical motions are not significantly affected by the substitution of the retinal's C-20 methyl group with an iodine atom. Molecular dynamics simulations indicate that a significant increase in free energy is associated with the 13-cis conformation of 13-I-pR, consistent with our observation that the transient 13-I-pR conformational state is reached more rapidly. We conclude that although the conformational trajectory is accelerated, the major transient conformation of pR is unaffected by the substitution of an iodinated retinal chromophore.

Author

Erik Malmerberg

University of Gothenburg

Ziad Omran

Jochen S Hub

Xuewen Li

Gergely Katona

University of Gothenburg

Sebastian Westenhoff

University of Gothenburg

Linda C Johansson

University of Gothenburg

Magnus Andersson

Chalmers, Chemical and Biological Engineering

Marco Cammarata

Michael Wulff

David van der Spoel

J Davidsson

Alexandre Specht

Richard Neutze

University of Gothenburg

Biophysical Journal

0006-3495 (ISSN) 1542-0086 (eISSN)

Vol. 101 6 1345-53

Subject Categories

Biochemistry and Molecular Biology

Biophysics

Structural Biology

DOI

10.1016/j.bpj.2011.07.050

PubMed

21943415

More information

Created

10/6/2017