Kinetic modeling of rapid enzymatic hydrolysis of crystalline cellulose after pretreatment by NMMO
Journal article, 2012

Pretreatment of cellulose with an industrial cellulosic solvent, N-methylmorpholine-N-oxide, showed promising results in increasing the rate of subsequent enzymatic hydrolysis. Cotton linter was used as high crystalline cellulose. After the pretreatment, the cellulose was almost completely hydrolyzed in less than 12 h, using low enzyme loading (15 FPU/g cellulose). The pretreatment significantly decreased the total crystallinity of cellulose from 7.1 to 3.3, and drastically increased the enzyme adsorption capacity of cellulose by approximately 42 times. A semi-mechanistic model was used to describe the relationship between the cellulose concentration and the enzyme loading. In this model, two reactions for heterogeneous reaction of cellulose to glucose and cellobiose, and a homogenous reaction for cellobiose conversion to glucose was incorporated. The Langmuir model was applied to model the adsorption of cellulase onto the treated cellulose. The competitive inhibition was also considered for the effects of sugar inhibition on the rate of enzymatic hydrolysis. The kinetic parameters of the model were estimated by experimental results and evaluated.

Enzymatic hydrolysis

N-Methylmorpholine-N-oxide

saccharification

biogas production

Kinetic modeling

binding

dilute-acid

Pretreatment

systems

conversion

Substrate reactivity

lignocellulosic biomass

fibers

ethanol

Author

M. Khodaverdi

University of Borås

Azam Jeihanipour

Chalmers, Chemical and Biological Engineering, Chemical Reaction Engineering

K. Karimi

University of Isfahan

Mohammad Taherzadeh Esfahani

University of Borås

Journal of Industrial Microbiology and Biotechnology

1367-5435 (ISSN) 1476-5535 (eISSN)

Vol. 39 3 429-438

Subject Categories

Industrial Biotechnology

DOI

10.1007/s10295-011-1048-y

More information

Latest update

3/14/2019