Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry.
Review article, 2011
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides a method for the detection of native and exogenous compounds in biological samples on a cellular scale. Through the development of novel ion beams the amount of molecular signal available from the sample surface has been increased. Through the introduction of polyatomic ion beams, particularly C(60), ToF-SIMS can now be used to monitor molecular signals as a function of depth as the sample is eroded thus proving the ability to generate 3D molecular images. Here we describe how this new capability has led to the development of novel instrumentation for 3D molecular imaging while also highlighting the importance of sample preparation and discuss the challenges that still need to be overcome to maximise the impact of the technique.
Cell Membrane
Spectrometry
analysis
Animals
methods
instrumentation
Xenopus laevis
Mass
Software
Humans
analysis
chemistry
Phospholipids
Three-Dimensional
Ions
methods
instrumentation
HeLa Cells
Amino Acids
instrumentation
Molecular Imaging
Secondary Ion
Imaging
Principal Component Analysis
methods
chemistry
Author
John Fletcher
John C Vickerman
Nicholas Winograd
Current opinion in chemical biology
1879-0402 (ISSN)
Vol. 15 5 733-40Subject Categories
Analytical Chemistry
DOI
10.1016/j.cbpa.2011.05.016
PubMed
21664172