Investigating the Yet-Unknown Biomass Degrading and Modifying Enzymes of Aspergillus oryzae
Conference poster, 2012

The association of plant cell wall components with A. oryzae’s extracellular enzyme machinery was investigated by analyzing the transcriptome profile in relation to the chemical structure of the carbon sources. Strain RIB40 of A. oryzae was cultured on various carbon sources, namely cellohexaose, mannohexaose, xylopentaose, arabinoheptaose, glucohexaose, glucosyl maltotriosyl maltotriose, galactosyl mannotriose, turanose and sophorose, and the transcribed genes were determined with DNA microarrays. The statistically significant genes were selected and potential novel hydrolases were identified. Presently, we aim at the heterologous expression and characterization of seven different hypothetical and non classified proteins of A. oryzae, which could prove to be useful tools in the wood biomass separation and modification process. This work represents a novel way of integrating computational chemical biology and classical enzyme research for improving lignocellulose bioconversion.

Author

George E Anasontzis

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Gianni Panagiotou

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Lisbeth Olsson

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Science and Technology Day, 27 March 2012, Göteborg, Sweden

Subject Categories

Biochemistry and Molecular Biology

Microbiology

Biocatalysis and Enzyme Technology

Areas of Advance

Energy

Life Science Engineering (2010-2018)

More information

Created

10/7/2017