Freeze-Drying as Sample Preparation for Micellar Electrokinetic Capillary Chromatography-Electrochemical Separations of Neurochemicals in Drosophila Brains
Journal article, 2013

Micellar electrokinetic capillary chromatography with electrochemical detection has been used to quantify biogenic amines in freeze-dried brains of Drosophila melanogaster. Freeze-drying samples offers a way to preserve the biological sample while making dissection of these tiny samples easier and faster. Fly samples were extracted in cold acetone and dried in a rotary evaporator. Extraction and drying times were optimized in order to avoid contamination by red pigment from the fly eyes and still have intact brain structures. Single freeze-dried fly brain samples were found to produce representative electropherograms as a single hand-dissected brain sample. With utilization of the faster dissection time that freeze-drying affords, the number of brains in a fixed homogenate volume can be increased to concentrate the sample. Thus, concentrated brain samples containing five or fifteen preserved brains were analyzed for their neurotransmitter content, and four analytes; N-acetyloctopamine, N-acetylserotonin, N-acetyltyramine, and N-acetyldopamine were found to correspond well with previously reported values.


E. C. Berglund

University of Gothenburg

N. J. Kuklinski

Pennsylvania State University

E. Karagunduz

University of Gothenburg

Kubra Ucar

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Jörg Hanrieder

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Andrew Ewing

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 85 5 2841-2846

Subject Categories

Analytical Chemistry



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4/6/2018 1