An Optofluidic Temperature Probe
Journal article, 2013

We report the application of a microfluidic device for semi-contact temperature measurement in picoliter volumes of aqueous media. Our device, a freely positionable multifunctional pipette, operates by a hydrodynamic confinement principle, i.e., by creating a virtual flow cell of micrometer dimensions within a greater aqueous volume. We utilized two fluorescent rhodamines, which exhibit different fluorescent responses with temperature, and made ratiometric intensity measurements. The temperature dependence of the intensity ratio was calibrated and used in a model study of the thermal activation of TRPV1 ion channels expressed in Chinese hamster ovary cells. Our approach represents a practical and robust solution to the specific problem of measuring temperature in biological experiments in vitro, involving highly localized heat generation, for example with an IR-B laser.

microthermometer

TRPV1

optofluidic

Rhodamine 6G

microfluidic device

temperature sensing

Rhodamine B

semi-contact

multifunctional pipette

Author

Ilona Wegrzyn

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Alar Ainla

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Gavin Jeffries

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Aldo Jesorka

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Sensors

1424-8220 (ISSN) 1424-3210 (eISSN)

Vol. 13 4 4289-4302

Subject Categories

Chemical Sciences

DOI

10.3390/s130404289

More information

Latest update

1/16/2019