3D Culturing and differentiation of SH-SY5Y neuroblastoma cells on bacterial nanocellulose scaffolds.
Journal article, 2014

A new in vitro model, mimicking the complexity of nerve tissue, was developed based on a bacterial nanocellulose (BNC) scaffold that supports 3D culturing of neuronal cells. BNC is extracellularly excreted by Gluconacetobacter xylinus (G. xylinus) in the shape of long non-aggregated nanofibrils. The cellulose network created by G. xylinus has good mechanical properties, 99% water content, and the ability to be shaped into 3D structures by culturing in different molds. Surface modification with trimethyl ammonium beta-hydroxypropyl (TMAHP) to induce a positive surface charge, followed by collagen I coating, has been used to improve cell adhesion, growth, and differentiation on the scaffold. In the present study, we used SH-SY5Y neuroblastoma cells as a neuronal model. These cells attached and proliferated well on the BNC scaffold, as demonstrated by scanning electron microscopy (SEM) and the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay. Following neuronal differentiation, we demonstrated functional action potentials (APs) by electrophysiological recordings, indicating the presence of mature neurons on the scaffolds. In conclusion, we have demonstrated for the first time that neurons can attach, proliferate, and differentiate on BNC. This 3D model based on BNC scaffolds could possibly be used for developing in vitro disease models, when combined with human induced pluripotent stem (iPS) cells (derived from diseased patients) for detailed investigations of neurodegenerative disease mechanisms and in the search for new therapeutics.

neuronal network 3D model

surface modification

scaffolds

SH-SY5Y cells

bacterial nanocellulose

Author

Marcus Innala

Chalmers, Chemical and Biological Engineering

Ilse Riebe

University of Gothenburg

Volodymyr Kuzmenko

Chalmers, Applied Physics, Electronics Material and Systems

Johan Sundberg

Chalmers, Chemical and Biological Engineering, Polymer Technology

Paul Gatenholm

Chalmers, Chemical and Biological Engineering, Polymer Technology

Eric Hanse

University of Gothenburg

Sara Johannesson

Chalmers, Chemical and Biological Engineering, Polymer Technology

Artificial Cells, Nanomedicine and Biotechnology

2169-1401 (ISSN) 2169-141X (eISSN)

Vol. 42 5 302-308

Subject Categories

Basic Medicine

DOI

10.3109/21691401.2013.821410

PubMed

23895194

More information

Created

10/8/2017