Polypeptide Multilayer Self-Assembly Studied by Ellipsometry
Journal article, 2014

A polypeptide nanofilm made by layer-by-layer (LbL) self-assembly was built on a surface that mimics nonwoven, a material commonly used in wound dressings. Poly-L-lysine (PLL) and poly-L-glutamic acid (PLGA) are the building blocks of the nanofilm, which is intended as an enzymatically degradable lid for release of bactericides to chronic wounds. Chronic wounds often carry infection originating from bacteria such as Staphylococcus aureus and a release system triggered by the degree of infection is of interest. The dry nanofilm was studied with ellipsometry. The thickness of the nanofilm was 60% less in its dry state than in its wet state. The measurements showed that a primer was not necessary to build a stable nanofilm, which is practically important in our case because a nondegradable primer is highly unwanted in a wound care dressing. Added V8 (glutamyl endopeptidase) enzymes only showed adsorption on the nanofilm at room temperature, indicating that the PLL/PLGA “lid” may remain intact until the dressing has been filled with wound exudate at the elevated temperature typical of that of the wound.

Author

Marina Craig

SuMo Biomaterials

Chalmers, Chemical and Biological Engineering

Krister Holmberg

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

E. Le Ru

P. Etchegoin

Journal of Drug Delivery

2090-3014 (ISSN) 2090-3022 (eISSN)

Vol. 2014 (article ID) 424697-

Subject Categories

Chemical Sciences

DOI

10.1155/2014/424697

More information

Latest update

8/18/2020