In vitro evaluation of human fetal osteoblast response to magnesium loaded mesoporous TiO2 coating.
Journal article, 2013

This work aimed to evaluate the in vitro response of Transfected Human Foetal Osteoblast (hFOB) cultured on a magnesium-loaded mesoporous TiO2 coating. The application of mesoporous films on titanium implant surfaces has shown very promising potential to enhance osseointegration. This type of coating has the ability to act as a framework to sustain bioactive agents and different drugs. Magnesium is the element that, after calcium, is the most frequently used to dope titanium implant surfaces, since it is crucial for protein formation, growth factor expression, and aids for bone mineral deposition on implant surfaces. Mesoporous TiO2 films with an average pore-size of 6 nm were produced by the evaporation-induced self-assembly method (EISA) and deposited onto titanium discs. Magnesium loading was performed by soaking the mesoporous TiO2 discs in a magnesium chloride solution. Surface characterization was conducted by SEM, XPS, optical interferometry, and AFM. Magnesium release profile was assessed at different time points using a Magnesium Detection kit. Cell morphology and spreading were observed with SEM. The cytoskeletal organization was stained with TRITC-conjugated Phalloidin and cell viability was evaluated through a mitochondrial colorimetric (MTT) assay. Furthermore, gene expression of bone markers and cell mineralization were analyzed by real time RT-PCR and alizarin-red staining, respectively. The surface chemical analysis by XPS revealed the successful adsorption of magnesium to the mesoporous coating. The AFM measurements revealed the presence of a nanostructured surface roughness. Osteoblasts viability and adhesion as well as the gene expression were unaffected by the addition of magnesium possibly due to its rapid burst release, however, were enhanced by the 3D nanostructure of the TiO2 layer.

magnesium

TiO2

osteoblast

Author

F. Cecchinato

Malmö university

Y. Xue

University of Bergen

Johan Karlsson

Applied Surface Chemistry

Wenxiao He

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Ann Wennerberg

Malmö university

K. Mustafa

University of Bergen

Martin Andersson

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Ryo Jimbo

Malmö university

Journal of biomedical materials research. Part A

1552-4965 (eISSN)

Vol. 102 11 3862-3871

Subject Categories

Dentistry

DOI

10.1002/jbm.a.35062

PubMed

24339394

More information

Latest update

12/27/2018