2-Butanol and Butanone Production in Saccharomyces cerevisiae through Combination of a B-12 Dependent Dehydratase and a Secondary Alcohol Dehydrogenase Using a TEV-Based Expression System
Journal article, 2014

2-Butanol and its chemical precursor butanone (methyl ethyl ketone -MEK) are chemicals with potential uses as biofuels and biocommodity chemicals. In order to produce 2-butanol, we have demonstrated the utility of using a TEV-protease based expression system to achieve equimolar expression of the individual subunits of the two protein complexes involved in the B-12-dependent dehydratase step (from the pdu-operon of Lactobacillus reuterii), which catalyze the conversion of meso-2,3-butanediol to butanone. We have furthermore identified a NADH dependent secondary alcohol dehydrogenase (Sadh from Gordonia sp.) able to catalyze the subsequent conversion of butanone to 2-butanol. A final concentration of 4 +/- 0.2 mg/L 2-butanol and 2 +/- 0.1 mg/L of butanone was found. A key factor for the production of 2-butanol was the availability of NADH, which was achieved by growing cells lacking the GPD1 and GPD2 isogenes under anaerobic conditions.

Author

PAYAM GHIACI

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Joakim Norbeck

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Christer Larsson

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

PLoS ONE

1932-6203 (ISSN)

Vol. 9 7 e102774

Subject Categories

Biological Systematics

DOI

10.1371/journal.pone.0102774

More information

Created

10/8/2017