ToF-SIMS imaging of lipids and lipid related compounds in Drosophila brain
Paper in proceedings, 2014

Drosophila melanogaster (fruit fly) has a relatively simple nervous system but possesses high order brain functions similar to humans. Therefore, it has been used as a common model system in biological studies, particularly drug addiction. Here, the spatial distribution of biomolecules in the brain of the fly was studied using time-of-flight SIMS. Fly brains were analyzed frozen to prevent molecular redistribution prior to analysis. Different molecules were found to distribute differently in the tissue, particularly the eye pigments, diacylglycerides, and phospholipids, and this is expected to be driven by their biological functions in the brain. Correlations in the localization of these molecules were also observed using principal components analysis of image data, and this was used to identify peaks for further analysis. Furthermore, consecutive analyses following 10keV Ar-2500(+) sputtering showed that different biomolecules respond differently to Ar-2500(+) sputtering. Significant changes in signal intensities between consecutive analyses were observed for high mass molecules including lipids.

SIMS

Drosophila

imaging mass spectrometry

lipids

Author

Nhu TN Phan

University of Gothenburg

John Fletcher

University of Gothenburg

Peter Sjövall

University of Gothenburg

Andrew Ewing

University of Gothenburg

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Surface and Interface Analysis

0142-2421 (ISSN) 1096-9918 (eISSN)

Vol. 46 S1 123-126

Subject Categories

Physical Chemistry

DOI

10.1002/sia.5547

More information

Created

10/7/2017