The supramolecular structure of cellulose-rich wood pulps can be a determinative factor for enzymatic hydrolysability
Journal article, 2015

The enzymatic hydrolysability of three industrial pulps, five lab made pulps, and one microcrystalline cellulose powder was assessed using commercial cellulolytic enzymes. To gain insight into the factors that influence the hydrolysability, a thorough characterization of the samples was done, including their chemical properties (cellulose content, hemicellulose content, lignin content, and kappa number), their macromolecular properties (peak molar mass, number-average molar mass, weight-average molar mass, polydispersity, and limiting viscosity) and their supramolecular properties (fibre saturation point, specific surface area, average pore size, and crystallinity). The hydrolysability was assessed by determination of initial conversion rate and final conversion yield, with conversion yield defined as the amount of glucose in solution per unit of glucose in the substrate. Multivariate data analysis revealed that for the investigated samples the conversion of cellulose to glucose was mainly dependent on the supramolecular properties, such as specific surface area and average pore size. The molar mass distribution, the crystallinity, and the lignin content of the pulps had no significant effect on the hydrolysability of the investigated samples.

Average pore size

Wood pulp

Cellulolytic enzymes

Specific surface area

Multivariate data analysis


F. Aldaeus


K. Larsson


J. Srndovic


M. Kubat


K. Karlstom


Ausra Peciulyte

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Lisbeth Olsson

Wallenberg Wood Science Center (WWSC)

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

P.T. Larsson



0969-0239 (ISSN)

Vol. 22 6 3991-4002

Subject Categories

Materials Engineering



More information

Latest update

9/6/2018 1