A lipid-based passivation scheme for nanofluidics
Paper in proceeding, 2012

Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.

Enzymatic Degradation

Bovine serum



Lipid bilayer

DNA cleavage

Nano channels

Single molecule

Single molecule level

Single molecules

Lipid bilayers



Protein-DNA interactions


Genomic DNA


Joachim Fritzsche

University of Gothenburg

F. Persson

University of Gothenburg

Uppsala University

K. U. Mir

Wellcome Trust Centre for Human Genetics

M. Modesti

Aix Marseille University

Fredrik Westerlund

Chalmers, Chemical and Biological Engineering, Physical Chemistry

J. O. Tegenfeldt

University of Gothenburg

16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012; Okinawa; Japan; 28 October 2012 through 1 November 2012

9780979806452 (ISBN)

Subject Categories

Physical Chemistry

Physical Sciences



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