Rapid determination of bulk microalgal biochemical composition by Fourier-Transform Infrared spectroscopy
Journal article, 2013

Analysis of bulk biochemical composition is a key in fundamental and applied studies of microalgae and is essential to understanding responses to different cultivation scenarios. Traditional biochemical methods for the quantification of lipids, carbohydrates and proteins are often time-consuming, often involve hazardous reagents, require significant amounts of biomass and are highly dependent on practitioner proficiency. This study presents a rapid and non-destructive method, utilising Fourier-Transform Infrared (FTIR) spectroscopy for the simultaneous determination of lipid, protein and carbohydrate content in microalgal biomass. A simple univariate regression was applied to sets of reference microalgal spectra of known composition and recognised IR peak integrals. A robust single-species model was constructed, with coefficients of determination r2>0.95, high predictive accuracy and relative errors below 5%. The applicability of this methodology is demonstrated by monitoring the time-resolved changes in biochemical composition of the marine alga Nannochloropsis sp. grown to nitrogen starvation. © 2013 Elsevier Ltd.

Microalgae

FTIR spectroscopy

Process monitoring

Nannochloropsis sp.

Lipids

Author

Joshua Mayers

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

K.J. Flynn

R.J. Shields

Bioresource Technology

0960-8524 (ISSN)

Vol. 148 215-220

Subject Categories

Analytical Chemistry

Bioprocess Technology

DOI

10.1016/j.biortech.2013.08.133

More information

Created

10/7/2017