X-radiation enhances the collagen type I strap formation and migration potentials of colon cancer cells
Journal article, 2016

Rectal cancer treatment still fails with local and distant relapses of the disease. It is hypothesized that radiotherapy could stimulate cancer cell dissemination and metastasis. In this study, we evaluated the effect of X-radiation on collagen type I strap formation potential, i.e. matrix remodeling associated with mesenchymal cell migration, and behaviors of SW480, SW620, HCT116 p53(+/+) and HCT116 p53(-/-) colon cancer cells. We determined a radiation-induced increase in collagen type I strap formation and migration potentials of SW480 and HCT116 p53(+/+). Further studies with HCT116 p53(+/+), indicated that after X-radiation strap forming cells have an increased motility. More, we detected a decrease in adhesion potential and mature integrin beta 1 expression, but no change in non-muscle myosin II expression for HCT116 p53(+/+) after X-radiation. Integrin beta 1 neutralization resulted in a decreased cell adhesion and collagen type I strap formation in both sham and X-radiated conditions. Our study indicates collagen type I strap formation as a potential mechanism of colon cancer cells with increased migration potential after X-radiation, and suggests that other molecules than integrin beta 1 and non-muscle myosin II are responsible for the radiation-induced collagen type I strap formation potential of colon cancer cells. This work encourages further molecular investigation of radiation-induced migration to improve rectal cancer treatment outcome.

matrix contraction

Cell Biology

radiotherapy

collagen type 1

cell migration

integrin beta 1

reorganization

Oncology

metastasis

invasion

X-radiation

p53

colorectal cancer

growth-factor

nonmuscle myosin-ii

Author

Stephanie Blockhuys

Chalmers, Biology and Biological Engineering, Chemical Biology

N. Liu

Linköping University

Nisha Rani Agarwal

Chalmers, Biology and Biological Engineering, Chemical Biology

Annika Enejder

Chalmers, Biology and Biological Engineering, Chemical Biology

V. Loitto

Linköping University

X. F. Sun

Linköping University

Oncotarget

1949-2553 (ISSN)

Vol. 7 44 71390-71399

DOI

10.18632/oncotarget.12111

More information

Latest update

2/28/2018