Ultrasonic-Aided Fabrication of Nanostructured Au-Ring Microelectrodes for Monitoring Transmitters Released from Single Cells
Journal article, 2017

We report a novel ultrasonic-aided fast and straightforward approach to fabricate Au microelectrodes by electroless deposition of nanostructured gold films on the rigid outer surface of pulled glass capillaries. Microelectrodes with tip diameters ranging from several hundred nanometers to several micrometers were fabricated within 20 min via three sequential ultrasonication steps. The ultrasonication technique has been validated to be a very effective route in engineering the morphology of Au film surfaces and improves the fabrication efficiency of Au microelectrodes. The nanostructured surfaces of the Au microelectrodes demonstrate excellent sensing activity and antifouling for dopamine oxidation. The microelectrodes were applied for measurement of catecholamines released from exocytosis events from single chromaffin cells and exhibited faster dynamic peak parameters, compared with carbon fiber microelectrodes. This report provides a generally accessible and complementary platform for analyzing catecholamines release events, which should be useful for new electrode designs and neurochemical sensing.

Author

Keqing Wang

Capital Normal University

Xu Zhao

Capital Normal University

Bo Li

Capital Normal University

Kai Wang

Chinese Academy of Sciences

Xin Zhang

Capital Normal University

Lanqun Mao

Chinese Academy of Sciences

Andrew Ewing

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Y. Q. Lin

Capital Normal University

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 89 17 8683-8688

Subject Categories

Chemical Sciences

DOI

10.1021/acs.analchem.7b02814

More information

Latest update

9/4/2018 1