Manufacturing and characterization of bacterial cellulose tubes using two different fermentation techniques
Book chapter, 2006

A modified defined media with 0.6% lactate resulted in high cellulose yield and was therefore used in the tube formation. Two fermentation techniques were compared, A and B, using a modified disc reactor. Scanning Electron Microscopy (SEM) showed that tubes from method A were more homogeneous with denser parts in the network. In contrast, the tubes grown according to method B have denser inner surface and a porous outer surface. Mechanical measurements could not be done on the tubes from method A due to their fragility. However the tubes from method B were measured and showed to be stiffer, stronger, and less extensible when compared with a BC pellicle. This can probably be explained by a slightly higher solid content of the tubes and presumably a more interconnected fibril network as a consequence. The BC tubes manufactured according to fermentation method B results in desired asymmetric structure and mechanical properties and are expected to be used as a scaffold for tissue engineered blood vessels.


Aase Katarina Bodin

Chalmers, Chemical and Biological Engineering, Molecular Biotechnology

Lena Gustafsson

Chalmers, Chemical and Biological Engineering, Molecular Biotechnology

Paul Gatenholm

Chalmers, Chemical and Biological Engineering, Polymer Technology

In: Modern multidisciplinary applied lmicrobiology; Exploiting microbes and their interactions/Ed A Medez-Vilas


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