Cell Membrane Derived Platform To Study Virus Binding Kinetics and Diffusion with Single Particle Sensitivity
Journal article, 2018

Discovery and development of new antiviral therapies essentially rely on two key factors: an in-depth understanding of the mechanisms involved in viral infection and the development of fast and versatile drug screening platforms. To meet those demands, we present a biosensing platform to probe virus-cell membrane interactions on a single particle level. Our method is based on the formation of supported lipid bilayers from cell membrane material. Using total internal reflection fluorescence microscopy, we report the contribution of viral and cellular components to the interaction kinetics of herpes simplex virus type 1 with the cell membrane. Deletion of gC, the main viral attachment glycoprotein, or deletion of heparan sulfate, an attachment factor on the cell membrane, leads to an overall decrease in association of virions to the membrane and faster dissociation from the membrane. In addition to this, we perform binding inhibition studies using the antiviral compound heparin to estimate its IC50 value. Finally, single particle tracking is used to characterize the diffusive behavior of the virus particles on the supported lipid bilayers. Altogether, our results promote this platform as a complement to existing bioanalytical assays, being at the interface between simplified artificial membrane models and live cell experiments.

single particle tracking

biomimetic sensing platform

herpes simplex virus

supported lipid bilayers

virus−membrane interactions

binding kinetics

Author

Nadia Peerboom

Chalmers, Physics, Biological Physics

Eneas Schmidt

Chalmers, Physics, Biological Physics

Edward Trybala

University of Gothenburg

Stephan Block

Freie Universität Berlin

Tomas Bergström

University of Gothenburg

Hudson Pace

Chalmers, Physics, Biological Physics

Marta Bally

Umeå University

Chalmers, Physics, Biological Physics

ACS Infectious Diseases

2373-8227 (eISSN)

Vol. 4 6 944-953

Probing multivalent interactions: from the membrane´s nanoscale dynamics to virus infection

Swedish Research Council (VR), 2013-01-01 -- 2016-12-31.

Subject Categories

Cell Biology

Biophysics

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1021/acsinfecdis.7b00270

More information

Latest update

7/4/2018 1