Effect of microbial transglutaminase and setting condition on gel properties of blend fish protein isolate recovered by alkaline solubilisation/isoelectric precipitation
Journal article, 2019

The effect of microbial transglutaminase (M‐TGase) (0–0.6 units g−1 sample) and setting condition (25 °C/180 min, 30 °C/120 min, 35 °C/60 min and 40 °C/30 min) on gel properties of blend protein isolate of gutted kilka and silver carp was studied. The protein isolate provided a good substrate for M‐TGase activity so that a low amount of M‐TGase (0.2 unit g−1 sample) substantially improved textural properties and water holding capacity (WHC) of the gels. Breaking force of the gels was positively affected by M‐TGase up to 0.6 unit g−1 sample, but it negatively affected their WHC. Prior setting at 25–35 °C increased the breaking force of proteins compared to directly heated gel, resulting in maximum breaking force at 35 °C/60 min. However, the setting at 40 °C/30 min caused proteolysis, which was reflected in higher amounts of TCA‐soluble peptides and gel weakening. Denser microstructure and higher myosin heavy chain polymerisation observed in the gels which experienced the setting was well correlated with improvement in textural properties.

pH-shift method

microbial transglutaminase

Fish protein isolate

Blend surimi

setting condition

Author

Mehdi Abdollahi

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Masoud Rezaei

Tarbiat Modares University

Ali Jafarpour

Sari Agricultural Sciences and Natural Resources University

Ingrid Undeland

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

International Journal of Food Science and Technology

0950-5423 (ISSN) 1365-2621 (eISSN)

Vol. 54 3 762-770

Driving Forces

Sustainable development

Innovation and entrepreneurship

Subject Categories

Biochemistry and Molecular Biology

Food Engineering

Biophysics

Areas of Advance

Life Science Engineering (2010-2018)

Materials Science

DOI

10.1111/ijfs.13991

More information

Latest update

4/15/2019